Human T-cell leukemia virus form 1 (HTLV-1) was present in 1980 as the first, and to date, the one retrovirus that causes human most cancers. Whereas HTLV-1 an an infection is normally asymptomatic, 3-5% of contaminated folks develop a T cell neoplasm usually referred to as grownup T cell leukemia/lymphoma (ATL) a few years after an an infection.
Since its discovery, HTLV-1 has served as a model for understanding retroviral oncogenesis, transcriptional regulation, cellular signal transduction, and cell-associated viral an an infection and unfold. Loads of the preliminary evaluation was focused on the viral trans-activator/oncoprotein, Tax. Over the earlier decade, the analysis of HTLV-1 has entered the genomic interval.
With the occasion of current methods for locating out HTLV-1 an an infection and pathogenesis, the completion of your complete genome, exome and transcriptome sequencing analyses of ATL, and the invention of HBZ as one different HTLV-1 oncogene, many established concepts about how HTLV-1 infects, persists and causes sickness have undergone substantial revision.
This chapter seeks to mix our current understanding of the mechanisms of movement of Tax and HBZ with the good genomic data of ATL to provide an abstract of how HTLV-1 infects, replicates and causes leukemia.
Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza An an infection
Capturing the dynamic replication and assembly processes of viruses has been hindered by the dearth of sturdy in situ hybridization (ISH) utilized sciences that permit delicate and simultaneous labeling of viral nucleic acid and protein. Customary DNA fluorescence in situ hybridization (FISH) methods are typically not acceptable with immunostaining.
We have attributable to this truth developed an imaging technique, MICDDRP (multiplex immunofluorescent cell-based detection of DNA, RNA and protein), which allows simultaneous single-cell visualization of DNA, RNA, and protein. Compared with commonplace DNA FISH, MICDDRP makes use of branched DNA (bDNA) ISH know-how, which dramatically improves oligonucleotide probe sensitivity and detection.
Small modifications of MICDDRP permit imaging of viral proteins concomitantly with nucleic acids (RNA or DNA) of varied strandedness. We have utilized these protocols to test the life cycles of quite a lot of viral pathogens, along with human immunodeficiency virus (HIV)-1, human T-lymphotropic virus (HTLV)-1, hepatitis B virus (HBV), hepatitis C virus (HCV), Zika virus (ZKV), and influenza A virus (IAV).
We demonstrated that we are going to successfully label viral nucleic acids and proteins all through a varied differ of viruses. These analysis can current us with improved mechanistic understanding of quite a lot of viral methods, and in addition to, perform a template for utility of multiplexed fluorescence imaging of DNA, RNA, and protein all through a broad spectrum of cellular methods.
Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) An an infection in People Dwelling With HIV/AIDS throughout the Pará State, Amazon Space of Brazil
The human T-lymphotropic virus (HTLV) is part of the group of retroviruses that share associated routes of transmission to the human immunodeficiency virus (HIV). Coinfection of these viruses can affect the medical course of every infections, and experiences have confirmed a sooner improvement to AIDS and the occasion of HIV-related opportunistic infections.
The current analysis investigated the demographic traits, prevalence, and the subtypes of HTLV amongst people dwelling with HIV/AIDS (PLWHA) throughout the State of Pará, Northern Brazil. Blood samples had been obtained from victims who had been attending a reference unit that provides medical assist to HIV-infected folks throughout the State of Pará, Brazil, via the interval of Might 2016 to June 2017.
Plasma samples had been screened by ELISA checks to detect antibodies anti-HTLV-1\/2. DNA and viral types had been acknowledged by real-time polymerase chain response (qPCR). All samples with viral DNA had been submitted to nested PCR and nucleotide sequencing. The overall coinfection payment was 1.4% (5/368), and all samples had been from subtype HTLV-1a.
No circumstances of HTLV-2 an an infection had been detected. The prevalence of HTLV-1 was bigger in females (80%), folks between 31 and 50 years of age, heterosexual, single, with low month-to-month income, with secondary tutorial stage or bigger, sporadic condom utilization, restricted number of sexual companions, and no historic previous of sexually transmitted infections.
All samples from HTLV-1-infected victims had been acknowledged as strains belonging to the subtype 1a (Cosmopolitan), subgroup A (Transcontinental). This analysis acknowledged that the prevalence of HIV/HTLV coinfection has dropped from eight to 1.3% throughout the current investigation.
There was a shift of HTLV subtype from a predominance of HTLV-2 an an infection to date to an exact solely HTLV-1a. There was no necessary affiliation between monetary, sociodemographic, and behavioral traits in HIV/HTLV coinfection.
Dietary Consumption and Serum Selenium Ranges Have an effect on the Finish results of HTLV-1 An an infection
Human T cell leukemia virus form 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP), as the most common neurological emersion related to HTLV-1, is a debilitating and lifelong treating sickness with no definitive treatment.
Furthermore, it has been determined that dietary compositions (inflammatory and anti-inflammatory) and some micronutrients (equal to vitamin D and selenium) have an effect on inflammatory and immune processes and with this background; the analysis was completed to match the dietary standing between age- and sex-matched with contaminated and non-infected HTLV-1.
In a multi-center setting, 70 healthful controls (HCs), 35 asymptomatic carriers (ACs), and 35 HAM/TSP victims had been recruited throughout the HTLV-1 Foundation, Ghaem Hospital, Mashhad Faculty of Medical Sciences, Mashhad, Iran. Dietary standing along with anthropometric indices, dietary (micro- and macronutrient) consumption, and serum vitamin D, vitamin B12, zinc, and selenium had been measured.
In anthropometric indices, suggest waist circumference (WC) throughout the service group was significantly bigger than the affected individual and the administration groups (p = 0.008). Inside the dietary consumption, the affected individual group obtained a lot much less energy, protein, mono-unsaturated fatty acids (MUFA), and oleic, nonetheless additional fat than the HTLV-1 service and administration groups, and these variations had been distinctive in three groups (p = 0.002, 0.005, 0.001, 0.01, and 0.001, respectively), whereas the service group obtained additional saturated fatty acid and fewer poly-unsaturated fatty acids (PUFA), linoleic, and linolenic than affected individual and administration groups with a definite necessary (p = 0.01, 0.007, 0.005, and 0.006, respectively) in three groups.
In micronutrient consumption, although selenium, zinc, and dietary nutritional vitamins B12 and D had been lower throughout the affected individual group than the service and administration group, nonetheless, no necessary variations had been observed. In contrast with micronutrient serum concentrations, dietary nutritional vitamins B12 and D and selenium throughout the affected individual group had been lower than the service and administration groups, nonetheless statistically, the considerable distinction was found solely throughout the selenium focus (p = 0.001).
 Mouse HTLV-1 ELISA Kit |
|
EMH0319 |
Abclonal |
96Tests |
EUR 521 |
 Rabbit Polyclonal antibody Anti-CRBN |
|
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
 Recombinant HTLV-1 Envelope |
|
7-07753 |
CHI Scientific |
100µg |
Ask for price |
Recombinant HTLV-1 Envelope |
|
7-07754 |
CHI Scientific |
500µg |
Ask for price |
Recombinant HTLV-1 Envelope |
|
7-07755 |
CHI Scientific |
1000µg |
Ask for price |
 Recombinant HTLV-1 Mosaic |
|
7-07773 |
CHI Scientific |
1000µg |
Ask for price |
 HTLV-1 Envelope Protein |
|
20-abx260142 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1372.00
|
|
- Shipped within 5-10 working days.
|
 HTLV-1 p24 Protein |
|
20-abx260271 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1609.00
|
|
- Shipped within 5-10 working days.
|
 HTLV-1 gp21 Protein |
|
20-abx260284 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1609.00
|
|
- Shipped within 5-10 working days.
|
 HTLV-1 gp46 Protein |
|
20-abx260285 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1609.00
|
|
- Shipped within 5-10 working days.
|
 HTLV-1 Mosaic Protein |
|
20-abx260286 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1609.00
|
|
- Shipped within 5-10 working days.
|
 HTLV 1 ELISA Kit |
|
VArk-Lsx012 |
Creative Biolabs |
1 KIT |
Ask for price |
|
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses. |
 Recombinant HTLV-1 Protein |
|
VAng-Lsx0308-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HTLV type 1, recombinant protein from E. coli. |
 Recombinant HTLV-1 p24 Core |
|
7-07756 |
CHI Scientific |
100µg |
Ask for price |
Recombinant HTLV-1 p24 Core |
|
7-07757 |
CHI Scientific |
500µg |
Ask for price |
Recombinant HTLV-1 p24 Core |
|
7-07758 |
CHI Scientific |
1000µg |
Ask for price |
 HTLV-1 gp21 recombinant protein |
|
00700-V-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
- Category: Antigens, HTLV, Ag
|
|
Description: HTLV-1 gp21 recombinant protein |
HTLV-1 gp21 recombinant protein |
|
00700-V-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
- Category: Antigens, HTLV, Ag
|
|
Description: HTLV-1 gp21 recombinant protein |
 HTLV-1 gp46 recombinant protein |
|
00701-V-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
- Category: Antigens, HTLV, Ag
|
|
Description: HTLV-1 gp46 recombinant protein |
HTLV-1 gp46 recombinant protein |
|
00701-V-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
- Category: Antigens, HTLV, Ag
|
|
Description: HTLV-1 gp46 recombinant protein |
 HTLV-1 p24 Core Protein |
|
20-abx260143 |
Abbexa |
-
EUR 885.00
-
EUR 342.00
-
EUR 1372.00
|
|
- Shipped within 5-10 working days.
|
 Human HTLV-1 ELISA Kit |
|
EHH0319 |
Abclonal |
96Tests |
EUR 521 |
 Bovine HTLV-1 ELISA Kit |
|
EBH0319 |
Abclonal |
96Tests |
EUR 521 |
 Canine HTLV-1 ELISA Kit |
|
ECH0319 |
Abclonal |
96Tests |
EUR 521 |
 Anserine HTLV-1 ELISA Kit |
|
EAH0319 |
Abclonal |
96Tests |
EUR 521 |
 Goat HTLV-1 ELISA Kit |
|
EGTH0319 |
Abclonal |
96Tests |
EUR 521 |
 Rat HTLV-1 ELISA Kit |
|
ERH0319 |
Abclonal |
96Tests |
EUR 521 |
 Rabbit HTLV-1 ELISA Kit |
|
ERTH0319 |
Abclonal |
96Tests |
EUR 521 |
 Porcine HTLV-1 ELISA Kit |
|
EPH0319 |
Abclonal |
96Tests |
EUR 521 |
 HTLV 1/2 ELISA Kit |
|
VArk-Lsx011 |
Creative Biolabs |
1 KIT |
Ask for price |
|
Description: Used for analyzing the presence of the human T-Cell lymphotropic viruses. |
 Recombinant HTLV-1 Mosaic Protein |
|
VAng-Lsx0310-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HTLV type 1 Mosaic Protein, recombinant protein from E. coli. |
 Recombinant HTLV-1 E Protein |
|
VAng-Lsx0321-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HTLV type 1 Envelope, recombinant protein from E. coli. |
 Polyclonal Goat anti-GST μ-form |
|
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST p-form |
|
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
) Anti-HTLV Type I gp46 Clone 65/6C2.2.34 (1 mg) |
|
0801094 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I gp46 Clone 65/6C2.2.34 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type I p19 Clone TP-7 (1 mg) |
|
0801107 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I p19 Clone TP-7 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type I p19 Clone 45/6.11.1.3 (1 mg) |
|
0801108 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I p19 Clone 45/6.11.1.3 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type I p24 Clone 46/3.24.4 (1 mg) |
|
0801110 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I p24 Clone 46/3.24.4 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type II p19 Clone 78/6.18.07 (1 mg) |
|
0801113 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type II p19 Clone 78/6.18.07 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type I gp46 Clone 68/4.11.21 (1 mg) |
|
0801118 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I gp46 Clone 68/4.11.21 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type II gp46 Clone 73/4.9.8 (1 mg) |
|
0801119 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type II gp46 Clone 73/4.9.8 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type II p24 Clone 75/4.21.11 (1 mg) |
|
0801120 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type II p24 Clone 75/4.21.11 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
) Anti-HTLV Type I gp46 Clone 67/5.5.13.1 (1 mg) |
|
0801127 |
Zeptometrix |
1 mg |
EUR 1689.04 |
- What is the product classification?
- Anti-HTLV Type I gp46 Clone 67/5.5.13.1 is marked as RUO.
|
|
Description: Please contact Gentaur in order to receive the datasheet of the product. |
 HTLV-1 virus mosaic recombinant antigen |
|
00205-V-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
- Category: Antigens, HTLV, Ag
|
|
Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
HTLV-1 virus mosaic recombinant antigen |
|
00205-V-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
- Category: Antigens, HTLV, Ag
|
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Description: HTLV-1 virus mosaic recombinant antigen a.a. 374-400/190-207. |
 Peptide) Tax9, HTLV 1 (11 19) Peptide |
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20-abx266138 |
Abbexa |
-
EUR 411.00
-
EUR 662.00
-
EUR 314.00
|
|
- Shipped within 5-10 working days.
|
 Guinea Pig HTLV-1 ELISA Kit |
|
EGH0319 |
Abclonal |
96Tests |
EUR 521 |
 Recombinant HTLV-1 P24 Core Protein |
|
VAng-Lsx0313-100g |
Creative Biolabs |
100 µg |
EUR 813 |
|
Description: HTLV type 1 P24 Core, recombinant protein from E. coli. |
Recombinant HTLV-1 P24 Core Protein |
|
VAng-Lsx0313-1mg |
Creative Biolabs |
1 mg |
EUR 3420 |
|
Description: HTLV type 1 P24 Core, recombinant protein from E. coli. |
 Recombinant HTLV-1 P24 Protein [His] |
|
VAng-Lsx0314-1mgEcoli |
Creative Biolabs |
1 mg (E. coli) |
EUR 939 |
|
Description: HTLV-1 P24 Protein [His], recombinant protein from E. coli. |
 Recombinant HTLV-1 gp 46 Protein |
|
VAng-Lsx0319-100g |
Creative Biolabs |
100 µg |
EUR 1844 |
|
Description: HTLV type 1 Glycoprotein 46, recombinant protein from E. coli. |
 Recombinant HTLV-1 E 701 Protein |
|
VAng-Lsx0322-50g |
Creative Biolabs |
50 µg |
EUR 1754 |
|
Description: HTLV type 1 Envelope 701, recombinant protein from E. coli. |
 Recombinant HTLV-1 gag p19 Protein |
|
VAng-Lsx7388-100g |
Creative Biolabs |
100 µg |
EUR 1828 |
|
Description: HTLV type 1 gag p19, recombinant protein from yeast. |
 HTLV-I Env. Protein |
|
abx060597-1mg |
Abbexa |
1 mg |
EUR 1344 |
- Shipped within 5-10 working days.
|
 HTLV I gp21 Protein |
|
abx060598-100ug |
Abbexa |
100 ug |
EUR 676 |
- Shipped within 5-10 working days.
|
 HTLV I gp46 Protein |
|
abx060599-100ug |
Abbexa |
100 ug |
EUR 676 |
- Shipped within 5-10 working days.
|
 HTLV I p24 Protein |
|
abx060600-1mg |
Abbexa |
1 mg |
EUR 1483 |
- Shipped within 5-10 working days.
|
 Recombinant HTLV P24 Protein |
|
VAng-Lsx0305-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HTLV P24, recombinant protein from E. coli. |
 Recombinant HTLV-2 Protein |
|
VAng-Lsx0309-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HTLV type 2, recombinant protein from E. coli. |
 (AA: Leu-Phe-Gly-Tyr-Pro-Val-Tyr-Val) (MW: 957.15)) Tax8, HTLV - 1 (12 - 19) (AA: Leu-Phe-Gly-Tyr-Pro-Val-Tyr-Val) (MW: 957.15) |
|
SP-86611-1 |
Alpha Diagnostics |
1 mg |
EUR 164 |
 ELISA Kit) Human T-Lymphotropic Virus 1 (HTLV-1) ELISA Kit |
|
abx052972-96tests |
Abbexa |
96 tests |
EUR 668 |
- Shipped within 5-10 working days.
|
 Anti-Periphilin 1 Antibody |
|
A06996-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse. |
 Anti-Lyl-1 Antibody |
|
A07491-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
 Anti-GLI-1 Antibody |
|
A07972-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Cerebellin 1 Antibody |
|
A09176-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-DOC-1 Antibody |
|
A09467-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
 Anti-NPDC-1 Antibody |
|
A12846-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-CNG-1 Antibody |
|
A05494-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Flk-1 Antibody |
|
A00901-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse. |
 Anti-EDG-1 Antibody |
|
A01502-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-ROBO-1 Antibody |
|
A01530-1 |
BosterBio |
100ug |
EUR 455 |
|
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-Bag-1 Antibody |
|
A02423-1 |
BosterBio |
50 ul |
EUR 397 |
|
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
 Anti-TUB 1 Antibody |
|
A02917-1 |
BosterBio |
100ug/vial |
EUR 294 |
The analysis confirmed that there have been variations in dietary consumption (along with energy, macronutrients, and fatty acids), WC, and selenium serum ranges between HAM/TSP victims and HTLV-1 carriers, suggesting that dietary statues have an effect on the inflammatory immune response in HTLV-1 an an infection.
)
)
 HTLV-1 Envelope)
 HTLV-1 gp21)
 HTLV-1 gp46)
 HTLV-1 p24 Core)
 HTLV-1 Mosaic)
