GelRed is a sensitive, stable, and environmentally safe fluorescent nucleic acid dye designed to replace highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA, or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have practically the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. Furthermore, GelRed is much more sensitive than EtBr (Figure 2).
The dye is not cytotoxic or mutagenic at concentrations well above the working concentrations used in gel staining. GelRed successfully passed environmental safety tests in accordance with CCR Title 22 Hazardous Waste Characterization, according to which GelRed is not classified as a hazardous waste.
Application
GelRed is a fluorescent nucleic acid stain designed to replace highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA, or RNA in agarose gels or polyacrylamide gels.
Spectral properties
Absorbance: Standard transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR, or GelStar filter
Storage and stability
GelRed is a very stable dye. Store GelRed at room temperature, protected from light. Dye precipitation can occur at lower temperatures, resulting in a lower signal or the appearance of a precipitate on the gel surface. If this occurs, heat the solution at 45-50 ° C for two minutes and vortex. Protect yourself from light.
Disclaimer
Unless otherwise noted in our catalog or other company documentation accompanying the product (s), our products are designed for research use only and are not to be used for any other purpose, including but not limited to commercial uses. unauthorized, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
