The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.
|Detection range||0.12-2.50 mmol/L|
|Detection method||Colorimetric method|
|Assay time||50 min|
|Precision||Average inter-assay CV: 9.9%Average intra-assay CV: 4.8%|
|Other instruments required||Micropipettor, Centrifuge, Incubator, Vortex mixer|
|Other reagents required||Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)|
|Valid period||6 months|
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-2.50 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|Sample type||Dilution factor|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
The EpiMag HT (96-Well) Magnetic Separator is a magnetic stand (magnetic rack) that allows paramagnetic bead precipitation of liquid samples from various flat-bottom or U-bottom 96-well microplates (we recommend our EpiMag 96-Well Microplates for ideal compatibility). It can be used for isolation and purification of nucleic acids and proteins, immunoprecipitation, immunoassays (ELISA), cell sorting, and purification of biomolecules. A PCR adapter is also included for the option of using V-bottom semi-skirted/unskirted PCR microplates. This magnetic separation device has the following features and advantages:
The EpiMag works well with all standard microplates, including one to two milliliter deep-well microplates. Plus, with the use of the included adaptor, can be used with various P.C.R. plates.
- Ultra-Powerful NdFeB Magnets
- Efficient Particle Washing
- Maximum Sample Concentration
- Universal Compatibility
- Lightweight Handheld Design
- Attractive Price
pack of 100 ea
well maximum volume
Features and Benefits
- BRAND 96-well microplate, U-bottom is a deep-well plate ideal for agglutination and other assays requiring stirring and washing of samples.
- Features a round-shaped well bottom with no edges.
- Raised rings around the orifice prevent possible cross-contamination.
- Compatible with a range of microplate centrifuges.
- Securely sealable with suitable sealing mats or self-adhesive sealing films.
- Alphanumeric code for accurate identification.
- Made from high-transparency medical grade polypropylene (PP) that facilitates sample visibility.
- Chemically resistant to most solvents including DMSO, phenol, and chloroform.
Choosing the right microplate is a critical, often overlooked, part of an assay. The right microplate helps provides valuable data, whereas the wrong microplate can lead to missed or inaccurate data leading to missed project timelines and ultimately higher costs.
New technologies for drug development are rapidly advancing. These technologies have given rise to a variety of applications that require their own unique consumables. At PerkinElmer, we provide microplates that help you discover better. Covering a vast array of application areas, PerkinElmer microplates are available in different well formats, plastic types, coatings, and colors – clear, white, black, gray. Our microplates have been engineered to deliver the highest quality data for applications ranging from high throughput screening to cellular imaging. Discover the difference a better microplate makes.
- Footprint dimensions meet the SBS industry standard, guaranteeing compatibility with microplate-based instrumentation
- Carefully monitored microplate planarity to ensure superb data and image acquisition
- Custom microplate services including barcoding, custom packaging, as well as plate surface coatings to fit your application
- Full range of validated reagents and instruments to complement microplates.
96 well plates are used in the laboratory to perform a number of chemical reactions as well as medical tests, including PCR. Therefore, such plates are very common. The plates are among the consumables when using deep well plates of the same format. The plates have standard widths and lengths to be compatible with other laboratory equipment.
Now you can download the 96 well plate template directly on this page to use it in your lab operations. Stay tuned to find out exactly what the plates are used for and how to use the template.
96 Well Template Description
96 well plates are also often called microplates, so do not confuse this name with another one; it means the same thing. Microplates are compatible with deep well plates that are used for different types of analyses. Most of them are aimed at collecting samples, developing chemical compounds, purifying nucleic acids, and so on. So, microplates that are quite often used in medical and chemical laboratories are compatible with 96 well templates.
To identify the data, the template has its own designations. The lines are marked with an alphanumeric code, such as A1, A1, and so on, up to H12. It allows you to navigate and not confuse the information when you use a 96 well plate. It also has lines for signing the date and identifying the laboratory experiment by the operation number.
What are 96 Well Plates Used for?
The 96 well template is used for the design of chemical laboratory experiments. For example, several rows of a deep well plate are seeded with different substances, and then the same substrate or element is added to each row. Next, the chemist looks at how the added element affects the substance and how it behaves in this environment under different conditions. The template helps to plan such a study, that is, to mark up in advance the compositions of substances that the chemist adds to the different rows of the deep well plate. Based on the results of the chemical reaction, it correlates the identified effects of various substrates on the main component that needed to be analyzed.
One of the most common cases of using 96 well plates, especially becoming popular this year, is the PCR method. The PCR is used for various applications and analyses when it is necessary to multiply (by copying) the amount of a sample of DNA or RNA. The tablets are ideally suited for applications where the optical signal of quantitative PCR (qPCR) is read through the bottom of the tablet.
When conducting PCR in a thermal cycler, you apply the tape to the PCR plate from above after you have filled the cells of the deep well plate. So you can get data on the state of the elements in each well of the deep well plate due to the translucency of the tape. Then you map the well plate to the template and enter the data into the latter. This is convenient to do owing to the fact that, as mentioned above, the template has an accurate markup and easily correlates with most standard deep well plates.
Other Charts and Tables
You can find even more editable charts and tables forms accessible here. Just below, we selected a number of the more popular forms available in this category. Also, do not forget that you can easily upload, fill out, and edit any PDF form at FormsPal.
- 52 Week Money Challenge
- 64 Team Double Elimination Bracket
- Check Register Template
- DnD Character Sheet
How to Complete 96 Well Template
Thanks to the simple markup and standardization, filling in the template is not difficult. You can print out the 96 well template and fill it out manually, as well as enter the data directly on the page in the PDF form. We offer you an online form builder that will help you conveniently complete and save the well template. Let’s see how to do this in three tips:
Identify the Experiment.
In order not to confuse the data for different experiments, first of all, specify the date and number of the experiment on the corresponding lines. You can also associate the template number with the number of the deep well plate.