Thyroid most cancers is a typical malignant tumour of the endocrine system and ranks ninth in most cancers incidence worldwide. An in depth physique of proof has demonstrated that lncRNAs play a crucial function within the development of thyroid most cancers.
The lncRNA MAPKAPK5-AS1 has been reported to be abnormally expressed and to play a task within the growth of varied human cancers. Nonetheless, MAPKAPK5-AS1’s potential function in thyroid most cancers development stays unknown.
The target of our research was to discover the function and mechanism of MAPKAPK5-AS1 in thyroid most cancers cells and supply a possible goal for its organic prognosis and therapy. We transfected sh-MAPKAPK5-AS1 and sh-NC into BCPAP and TPC-1 cells for loss-of-function assays.
Outcomes of RT-qPCR evaluation demonstrated that MAPKAPK5-AS1 was extra extremely expressed in thyroid most cancers cells in comparison with regular cells. Useful assays demonstrated that interfering with the expression of MAPKAPK5-AS1 notably repressed proliferation and invasion and accelerated apoptosis of BCPAP and TPC-1 cells.
Mechanistically, we discovered that miR-519e-5p was negatively regulated by MAPKAPK5-AS1 and that tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta (YWHAH) was a goal of miR-519e-5p.
Moreover, rescue assays demonstrated that downregulation of MAPKAPK5-AS1 expression inhibited cell proliferation, migration, and invasion and promoted apoptosis by sponging miR-519e-5p, thereby growing YWHAH expression.
Finally, our research revealed that MAPKAPK5-AS1 promotes proliferation and migration of thyroid most cancers cells by concentrating on the miR-519e-5p/YWHAH axis, which gives novel perception into the event and development of thyroid most cancers.

Vital reductions in apoptosis-related proteins (HSPA6, HSPA8, ITGB3, YWHAH, and PRDX6) are concerned in immune thrombocytopenia

To research variations within the expression of plasma proteins in immune thrombocytopenia (ITP) and regular management teams, bone marrow samples have been collected from 20 lively ITP sufferers and 20 wholesome controls.
Quantitative proteomics evaluation based mostly on mass spectrometry was used to measure the protein ranges and perceive the protein networks. We discovered differentially expressed proteins in ITP sufferers and wholesome controls.
Parallel response monitoring (PRM), a focused proteome quantification approach, was used to quantitatively verify the recognized goal proteins and confirm the proteomics knowledge. On this research, a complete of 829 proteins have been recognized, and the fold-change cut-off was set at 1.5 (sufferers vs controls); a complete of 26 proteins have been upregulated, and 69 proteins have been downregulated.
The bioinformatics evaluation indicated that some differentially expressed proteins have been related to apoptosis. KEGG enrichment evaluation confirmed that the apoptosis-related proteins have been intently associated to the PI3K-Akt signalling pathway.
PRM demonstrated that apoptosis-related proteins have been considerably decreased in ITP sufferers, which additional confirmed the essential impact of apoptosis on ITP pathogenesis. We hypothesised that apoptosis could also be intently associated to ITP pathogenesis by the PI3K-Akt signalling pathway.

MiR-660-5p promotes the development of hepatocellular carcinoma by interplay with YWHAH by way of PI3K/Akt signaling pathway

Presently, an increasing number of research present that aberrantly expressed microRNAs (miRNAs) are essential driving components for the pathogenesis of hepatocellular carcinoma (HCC).
Based mostly on the TCGA and GEO databases, miR-660-5p was recognized as a potential goal for HCC on this research.In HCC tissues, miR-660-5pexpressionwasparticularly excessive, and this was confirmed inHCC cell traces.
The upregulatedmiR-660-5p confirmed correlations with tumor dimension, tumor quantity, TNM stage and histological grade. In vitro experimental knowledge, aswellas in vivo proof confirmed that miR-660-5p has the flexibility to considerably improve the cell proliferation fee, clone formation, migration, invasion, and tumorigenic capability of HCC cells.
YWHAH is validated that focused by miR-660-5p utilizing twin luciferase reporter assay. Knockdown of YWHAH has been proven to partially reverse the tumor suppressive operate of miR-660-5p inhibitor.
Moreover, miR-660-5p/YWHAH axis might activate PI3K/AKT pathway, which promoted EMT and cell cycle processes. In conclusion, this research illustrated the operate of miR-660-5p/YWHAH axis in HCC and offered potential targets for treating HCC.

Lack of operate of Ywhah in mice induces deafness and cochlear outer hair cells’ degeneration.

In vertebrates, 14-3-Three proteins kind a household of seven extremely conserved isoforms with chaperone exercise, which bind phosphorylated substrates largely concerned in regulatory and checkpoint pathways.
IncRNA MAPKAPK5-AS1 promotes proliferation and migration of thyroid cancer cell lines by targeting miR-519e-5p/YWHAH
14-3-Three proteins are probably the most ample protein within the mind and are abundantly discovered within the cerebrospinal fluid in neurodegenerative illnesses, suggesting a crucial function in neuron physiology and demise.
Right here we present that 14-3-3eta-deficient mice displayed auditory impairment accompanied by cochlear hair cells’ degeneration. We present that 14-3-3eta is very expressed within the outer and interior hair cells, spiral ganglion neurons of cochlea and retinal ganglion cells.
Screening of YWHAH, the gene encoding the 14-3-3eta isoform, in non-syndromic and syndromic deafness, revealed seven non-synonymous variants by no means reported earlier than.
Amongst them, two have been predicted to be damaging in households with syndromic deafness. In vitro, variants of YWHAH induce delicate mitochondrial fragmentation and extreme susceptibility to apoptosis, in settlement with a lowered capability of mutated 14-3-3eta to bind the pro-apoptotic Unhealthy protein.
This research demonstrates that YWHAH variants can have a considerable impact on 14-3-3eta operate and that 14-3-3eta could possibly be a crucial issue within the survival of outer hair cells.

A polymorphism within the YWHAH gene encoding 14-3-Three eta that isn’t related to sporadic Creutzfeldt-Jakob illness (CJD).

14-3-Three proteins are abundantly expressed within the mind, significantly neuronal tissue and are thought to serve a number of organic features concerned in neuronal growth and cell progress and demise.
Latest research have proven associations of 14-3-Three genes with neurodegenerative issues based mostly on their chromosomal linkage to those illnesses and to regulatory features for the nervous system.
Though the function of 14-3-Three proteins within the pathogenesis of prion illnesses stays unknown, the detection of altered ranges of isoforms of the 14-3-Three protein within the cerebrospinal fluid is taken into account a biomarker for prognosis of sporadic Creutzfeldt-Jakob illness (sCJD).
To establish different susceptibility genes for prion illness, we examined nucleotide variations in YWHAH, a gene encoding 14-3-Three eta. This case-control research included 182 sCJD sufferers and 206 wholesome Koreans. Polymerase chain response was used to amplify open studying body and a few 3′-untranslated area (UTR) in exon 2, and direct sequencing was carried out.
One polymorphism, 753 G/A, was detected within the 3′-UTR of exon 2 on the YWHAH. The genotype distribution and allele frequencies of the YWHAH 753 G/A polymorphism weren’t considerably totally different between controls and sCJD sufferers.
This discovering signifies that YWHAH 753 G/A polymorphism is unlikely to be linked to genetic susceptibility or have a modifying impact in sCJD.

YWHAH antibody

70R-10462 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal YWHAH antibody

YWHAH antibody

70R-21363 50 ul
EUR 435
Description: Rabbit polyclonal YWHAH antibody

YWHAH Polyclonal Antibody

A53158 100 µg
EUR 570.55
Description: kits suitable for this type of research

YWHAH Polyclonal Antibody

31666-100ul 100ul
EUR 252

YWHAH Polyclonal Antibody

31666-50ul 50ul
EUR 187

YWHAH Rabbit pAb

A9079-100ul 100 ul
EUR 308

YWHAH Rabbit pAb

A9079-200ul 200 ul
EUR 459

YWHAH Rabbit pAb

A9079-20ul 20 ul
EUR 183

YWHAH Rabbit pAb

A9079-50ul 50 ul
EUR 223

Anti-YWHAH antibody

STJ116342 100 µl
EUR 277
Description: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

anti- YWHAH antibody

FNab09576 100µg
EUR 548.75
Description: Antibody raised against YWHAH

Anti-YWHAH antibody

PAab09576 100 ug
EUR 386

pSV40- Ywhah- m

PVT11618 2 ug
EUR 273

YWHAH Blocking Peptide

33R-1091 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HSPB8 antibody, catalog no. 70R-2328

Mouse YWHAH shRNA Plasmid

  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Rat YWHAH shRNA Plasmid

  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human YWHAH shRNA Plasmid

  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

YWHAH Antibody, HRP conjugated

  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against YWHAH. Recognizes YWHAH from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

YWHAH Antibody, FITC conjugated

  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against YWHAH. Recognizes YWHAH from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

YWHAH Antibody, Biotin conjugated

  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against YWHAH. Recognizes YWHAH from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

YWHAH Polyclonal Conjugated Antibody

C31666 100ul
EUR 397

YWHAH Recombinant Protein (Human)

RP108080 100 ug Ask for price

YWHAH Recombinant Protein (Rat)

RP237788 100 ug Ask for price

YWHAH Recombinant Protein (Mouse)

RP186107 100 ug Ask for price

YWHAH Polyclonal Antibody, HRP Conjugated

A53159 100 µg
EUR 570.55
Description: fast delivery possible

YWHAH Polyclonal Antibody, FITC Conjugated

A53160 100 µg
EUR 570.55
Description: reagents widely cited

YWHAH Polyclonal Antibody, Biotin Conjugated

A53161 100 µg
EUR 570.55
Description: Ask the seller for details

YWHAH ELISA Kit (Human) (OKCA00594)

OKCA00594 96 Wells
EUR 833
Description: Description of target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.156 ng/mL

YWHAH ORF Vector (Human) (pORF)

ORF036028 1.0 ug DNA
EUR 405

Ywhah ORF Vector (Mouse) (pORF)

ORF062037 1.0 ug DNA
EUR 506

Ywhah ORF Vector (Rat) (pORF)

ORF079264 1.0 ug DNA
EUR 506

14-3-3 Eta (YWHAH) Antibody

  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

14-3-3 Eta (YWHAH) Antibody

  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

14-3-3 Eta (YWHAH) Antibody

  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

YWHAH sgRNA CRISPR Lentivector set (Human)

K2657701 3 x 1.0 ug
EUR 339

Ywhah sgRNA CRISPR Lentivector set (Rat)

K6882801 3 x 1.0 ug
EUR 339

Ywhah sgRNA CRISPR Lentivector set (Mouse)

K4543101 3 x 1.0 ug
EUR 339

Monoclonal YWHAH / 14-3-3 Eta Antibody

AMM08537G 0.05ml
EUR 484
Description: A Monoclonal antibody against Human YWHAH / 14-3-3 Eta. The antibodies are raised in Mouse. This antibody is applicable in WB and IHC-P, IF

Monoclonal YWHAH Antibody (monoclonal) (M04), Clone: 6A12

AMM08539G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human YWHAH (monoclonal) (M04). The antibodies are raised in mouse and are from clone 6A12. This antibody is applicable in WB, E

Human 14-3-3 protein eta (YWHAH)

  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human 14-3-3 protein eta(YWHAH),partial expressed in E.coli

YWHAH sgRNA CRISPR Lentivector (Human) (Target 1)

K2657702 1.0 ug DNA
EUR 154
On evaluation stratified by the prion protein gene 129 or 219 genotype, no important relation was present in genotype and allele frequencies of the YWHAH 753G/A. That is the primary genetic affiliation research of YWHAH with sCJD populations.


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