With business house journey on the horizon, it is very important perceive how the microgravity setting of house results bone energy. The discount in skeletal loading is understood to trigger a speedy loss in bone density.
How this corresponds to losses of bone energy isn’t well-known, particularly when mixed with the osteoporotic results of growing old. On this examine, a mouse mannequin of hind limb suspension (HLS) was used to simulate the consequences of gravitational unloading. This was mixed with soluble receptor activator of nuclear issue kappa beta ligand (sRANK-L), which simulates age associated osteoporosis.
The proximal area of the tibia in mouse legs was scanned in-vivo pre-treatment in addition to on the conclusion of the examine with excessive decision micro computed tomography (µCT).
Topic particular finite component (FE) fashions had been constructed from these 3D pictures to evaluate bone energy by simulating mechanical loading on these bone microstructures. Parameters indicative of bone energy obtained from the FE fashions had been bone quantity, stiffness, structural effectivity, and the 10th and 90th percentile nodal Von-Mises Stresses.
Moreover, a mannequin sensitivity evaluation was carried out to evaluate how these parameters different with modifications in anatomic mannequin top. With regard to FE stiffness, HLS resulted in a 31% decline, sRANK-L resulted in a 16.8% decline, and HLS mixed with sRANK-L (HLS+sRANK-L) resulted in a 38.6% decline.
One attention-grabbing discovering is that HLS triggered a discount in each bone stiffness and bone structural effectivity, whereas sRANK-L didn’t trigger modifications in bone structural effectivity, suggesting the significance of skeletal loading for sustaining bone well being.
As well as, sRANK-L mixed with HLS triggered an extra decline in bone stiffness, however didn’t additional alter bone structural effectivity. In conclusion, this examine reveals that relying on the reason for osteoporosis, bone energy modifications usually are not essentially proportional to bone density modifications.
Thus, it is very important develop new scientific bone assessments past the present bone density measurement.Medical Relevance- These parameters are related to the microstructural mechanics of bone, and understanding how energy is decreased on a structural degree might result in the event of in-vivo bone energy testing clinically.
Receptor Activator of Nuclear Transcription Issue NF-κB (RANK), Its Ligand RANKL, and Pure Inhibitor of RANKL Osteoprotegerin (OPG) within the Blood Serum of Sufferers with Major Bone Tumors.
The content material of elements of the RANK/RANKL/OPG system, the important thing regulator of homeostasis within the bone tissue, in blood serum samples from 199 sufferers with main bone neoplasms and 131 virtually wholesome volunteers was measured by ELISA.
Borderline giantcell tumor of the bone with excessive osteoclastogenic and osteolytic exercise is characterised by a rise within the degree of all elements of this method and highest ratio of sRANKL/OPG within the blood serum. Research indexes in sufferers with numerous benign neoplasms and tumor-like bone lesions had been decrease than in sufferers with giant-cell tumor.
The sufferers with malignant bone tumors may very well be divided into 2 subgroups with reverse indexes of the RANK/RANKL/OPG system.
The sufferers with osteosarcoma and Ewing sarcoma had a low degree of sRANK, however a excessive degree of sRANKL.
The sufferers with chondrosarcoma and chordoma had a excessive degree of sRANK, however a low degree of sRANKL.
Recombinant soluble receptor activator of nuclear factor-kappaB inhibits parathyroid hormone-induced osteoclastogenesis in vitro.
The latest identification of receptor activator of nuclear factor-kappaB ligand (RANKL)/RANK/osteoprotegerin (OPG) cytokine system has led to a brand new molecular perspective on osteoclast biology and bone homeostasis.
Particularly, the interplay between RANKL and RANK is answerable for osteoclast differentiation. Within the current examine, we evaluated whether or not soluble RANK (sRANK) might act as an antagonist of RANKL and down-regulate osteoclastogenesis and bone resorption in vitro.
The prokaryotic expression vector coding for sRANK was constructed. Then the assemble was launched into E. coli Origami B (DE3) competent cells and recombinant sRANK was efficiently produced and purified by way of affinity chromatography.
sRANK decreased osteoclast-like cell (OLC) formation and resorption pit formation induced by parathyroid hormone (PTH) in a dose-dependent method. As well as, sRANK considerably inhibited PTH-induced mRNA expression of carbonic anhydrase II and tartrate-resistant acid phosphatase in murine bone marrow cells as confirmed by utilizing semi-quantitative RT-PCR.
The down-regulation was extremely correlated with the impact of sRANK on OLC formation from marrow cells. These information exhibit the anti-resorptive results of sRANK in vitro and spotlight the potential of sRANK as a novel therapeutic method to bone problems characterised by enhanced bone resorption.
Plasma and drainage fluid ranges of soluble receptor activator of nuclear factor-kB (sRANK), soluble receptor activator of nuclear factor-kB ligand (sRANKL) and osteoprotegerin (OPG) throughout proximal humerus fracture therapeutic.
Fracture therapeutic is an ordered course of that restores the structural integrity of the bone. Soluble receptor activator of nuclear factor-kB (sRANK), its ligand (sRANKL) and osteoprotegerin (OPG) are concerned in bone remodelling, thus they might play a task in fracture restore.
OPG, soluble RANK and RANKL ranges had been measured in plasma and in drainage fluid, collected from pre-surgery section to therapeutic in ten sufferers of each genders (age vary 26-65 years) with proximal humerus fracture needing osteosynthesis.
All sufferers confirmed fracture therapeutic. No important modifications within the concentrations of sRANKL and OPG had been noticed, whereas sRANK confirmed a major enhance in drainage fluid 24 hours post-surgery in contrast with intra-surgery time.
OPG ranges had been increased in plasma and drainage fluid than sRANK and sRANKL at every time level. Since there are not any revealed information about sRANK involvement in fracture therapeutic, our examine represents the primary preliminary indication a few native enhance of this marker focus instantly after surgical procedure.
Soluble receptor activator of nuclear issue kappaB Fc diminishes prostate most cancers development in bone.
Prostate most cancers (CaP) develops metastatic bone lesions that encompass a mix of osteosclerosis and osteolysis. We now have beforehand demonstrated that focusing on receptor activator of nuclear issue kappaB ligand (RANKL) with osteoprotegerin (OPG) prevents the osteolytic exercise of CaP and its capacity to ascertain tumor in bone.
Nevertheless, OPG can block tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis, suggesting that the scientific use of OPG might stop apoptosis of tumors mediated by TRAIL.
Thus, strategies to dam RANKL exercise, aside from OPG, could also be essential. Accordingly, we evaluated the flexibility of soluble murine RANK-Fc (sRANK-Fc) to forestall development of established CaP in a extreme mixed immunodeficient mouse implanted with fetal human bone.
We first confirmed that sRANK didn’t block TRAIL-mediated apoptosis of LuCaP cells in vitro and that it did block LuCaP-conditioned media-induced osteoclastogenesis in vitro. Then, LuCaP 35 CaP cells had been injected into the marrow house of the bone implanted within the extreme mixed immunodeficient mice implanted with fetal human bone and allowed to turn into tumors for six weeks.
Both automobile or sRANK-Fc was then administered for six weeks. sRANK-Fc diminished tumor-induced osteoblastic lesions as demonstrated by radiograph, bone mineral density measurement, and bone histomorphometry.
sRANK-Fc additionally decreased systemic bone transforming markers, together with serum osteocalcin and bone-specific alkaline phosphatase and urine N-telopeptide of collagen. Lastly, sRANK-Fc decreased serum prostate-specific antigen ranges and tumor quantity within the bone, which signifies decreased tumor burden.
sRANK Ligand, human recombinant |
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| 4318-50 | Biovision | each | EUR 744 |
sRANK Ligand, murine recombinant |
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| 4557-10 | Biovision | each | EUR 307.2 |
sRANK Ligand, murine recombinant |
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| 4557-1000 | Biovision | each | EUR 5866.8 |
sRANK Ligand, murine recombinant |
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| 4557-50 | Biovision | each | EUR 744 |
sRANK Ligand Polyclonal Antibody |
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| 5557-100 | Biovision | each | EUR 379.2 |
sRANK Ligand Polyclonal Antibody |
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| 5557-30T | Biovision | each | EUR 175.2 |
sRANK Receptor, human recombinant |
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| 7193-10 | Biovision | each | EUR 157.2 |
sRANK Receptor, human recombinant |
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| 7193-50 | Biovision | each | EUR 314.4 |
Polyclonal sRANK Ligand Antibody |
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| APR06425G | Leading Biology | 0.1 mg | EUR 790.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human sRANK Ligand . This antibody is tested and proven to work in the following applications: |
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Polyclonal sRANK Ligand Antibody |
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| APR00265G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human sRANK Ligand . This antibody is tested and proven to work in the following applications: |
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sRANK Ligand Recombinant Protein |
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| 40-691 | ProSci | 2 ug | EUR 311.1 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells,including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. CHO cell-derived Recombinant Human sRANK Ligand is a 22.1 kDa polypeptide comprised of the TNF-homologous region of RANKL and a N-terminal His-tag. |
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Recombinant Rat sRANK Ligand Protein |
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| PROTQ9ESE2 | BosterBio | 10ug | EUR 380.4 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues). |
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Recombinant Human sRANK Ligand Protein |
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| PROTO14788-3 | BosterBio | 10ug | EUR 380.4 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant human sRANKL is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues). |
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Recombinant Murine sRANK Ligand Protein |
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| PROTO35235-1 | BosterBio | 10ug | EUR 380.4 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant murine sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues). |
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Recombinant Human sRANK Receptor Protein |
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| PROTQ9Y6Q6-2 | BosterBio | 100ug | EUR 380.4 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cell-mediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.3 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (175 amino acid residues). |
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Polyclonal sRANK Ligand Polyclonal Antibody |
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| APR00294G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human sRANK Ligand Polyclonal . This antibody is tested and proven to work in the following applications: |
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sRank Ligand Recombinant Protein (Animal Free) |
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| 40-758 | ProSci | 2 ug | EUR 323.7 |
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Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANKL is a 20.0 kDa polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues). |
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Human CellExp™ sRANK Ligand, Human Recombinant |
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| P1552-10 | Biovision | 10 µg | EUR 211.2 |
Human CellExp™ sRANK Ligand, Human Recombinant |
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| P1552-50 | Biovision | 50 µg | EUR 636 |
sRANK Ligand ELISA Kit (Human) : 96 Wells (OKAG00062) |
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| OKAG00062 | Aviva Systems Biology | 96 Wells | EUR 715.2 |
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Description: Description of target: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 63 pg/mL |
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sRANK Ligand ELISA Kit (Mouse) : 96 Wells (OKAG00100) |
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| OKAG00100 | Aviva Systems Biology | 96 Wells | EUR 715.2 |
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Description: Description of target: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.;Species reactivity: Mouse;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 62.5 pg/mL |
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sRANK Soluble RANK Receptor Human Recombinant Protein |
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| PROTQ9Y6Q6 | BosterBio | Regular: 100ug | EUR 380.4 |
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Description: sRANK Receptor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 19.1kDa. ;The sRANK Receptor is purified by proprietary chromatographic techniques. |
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Human Soluble Receptor Activator of Nuclear Factor-kB, sRANK ELISA Kit |
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| CSB-E13539h-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
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Description: Quantitativesandwich ELISA kit for measuring Human Soluble Receptor Activator of Nuclear Factor-kB, sRANK in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human Soluble Receptor Activator of Nuclear Factor-kB, sRANK ELISA Kit |
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| 1-CSB-E13539h | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Soluble Receptor Activator of Nuclear Factor-kB, sRANK in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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ELISA kit for Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) |
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| KTE60225-48T | Abbkine | 48T | EUR 424.8 |
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Description: Quantitative sandwich ELISA for measuring Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) |
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| KTE60225-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2702.4 |
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Description: Quantitative sandwich ELISA for measuring Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) |
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| KTE60225-96T | Abbkine | 96T | EUR 686.4 |
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Description: Quantitative sandwich ELISA for measuring Human Soluble Receptor Activator of Nuclear Factor-kB (sRANK) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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Rat SRANKL ELISA Kit |
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| ERS0291 | Abclonal | 96Tests | EUR 625.2 |
Rat sRANKL ELISA Kit |
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| ELA-E0470r | Lifescience Market | 96 Tests | EUR 1063.2 |
Goat SRANKL ELISA Kit |
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| EGTS0291 | Abclonal | 96Tests | EUR 625.2 |
Mouse SRANKL ELISA Kit |
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| EMS0291 | Abclonal | 96Tests | EUR 625.2 |
In distinction, sRANK-Fc had no impact on s.c. implanted LuCaP cells. We conclude that sRANK-Fc is an efficient inhibitor of RANKL that diminishes development of CaP progress in bone by way of inhibition of bone transforming.
